Department of Cellular Physiology
print


Breadcrumb Navigation


Content

Group: Techniques

  • Optical Tweezers

    trap2

    Optical tweezers are an all-optical non-contact tool that can be combined with high-resolution light microscopy to measure pico-Newton forces and nanometre displacements of micron-sized refractile objects. This parameter range is suitable for studying the production of force by, and movement of, motor proteins in a quantitative fashion. more

  • TIRF

    tirf12

    For the fluorescent imaging of single molecules in vitro and in cells, probes need to be attached to molecules of interest. Probes can be synthetic fluorophores or recombinant fluorescent fusion proteins, such as green-fluorescent protein (GFP) and related proteins, which are co-expressed with the protein molecules, or fluorescent semi-conductor nano-particles (quantum dots). Alternatively, non-fluorescent particles that scatter light, such as colloidal gold or silver particles, have been used.

    While non-fluorescent, scattering labels can be imaged with dark-field microscopy, fluorescent probes are mostly visualised with total-internal-reflection fluorescence (TIRF) microscopy to reduce background and improve the signal-to-noise ratio. more