Methods
In our lab we apply a various number of different biochemical, functional and microscopic techniques:
- TIRF: Total internal reflection fluorescence microscopy
- STORM: Stochastic optical reconstruction microscopy
- PALM: Photo activated localization microscopy
- FRET: Förster resonance energy transfer
- FRAP: Fluorescence recovery after photobleaching
- Single Molecule Fluorescence
- Tryptophan fluorescence
- SPA: Single particle analysis
- SLiC: Sequence and ligation independent cloning
- Cell Transfection
- Protein expression
- Titration studies
- Immunoblots
- MST: Microscale thermophoresis
- SEC: Size-exclusion chromatography
- HPLC: High-performance liquid chromatography
- SLS: Static light scattering
- DLS: Dynamic light scattering
- Optical Tweezer
- Confocal Microscopy
- Functionality Assays
- Inverted Motility Assay